1704150-美国BIO-RAD伯乐 Trans-Blot® Turbo™ *型蛋白转印系统

Trans-Blot Turbo转印系统*结合了传统转印技术与现代印迹膜及滤纸合，可在zui短的时间内完成蛋白质从凝胶到膜的转印，且操作非常简便。系统内置了高电流电源， 可直接控制钛阳极和不锈钢阴极间的电流，能在3分钟内实现高效快速转印。全套系统采取即用型设计，研究人员可更快、更轻松的获得结果，并且具有传统印迹方 法难以实现的高重复性。

• 快速转印 — 只需 3 分钟即可转印小型凝胶或中型凝胶
• 高通量 — 单次运行可转印 1–4 块小型胶或 1–2 块中型胶
• 更高的转印效率 — 比其他转印方法的转印效率更高
• 灵活设计 — 允许用户自定义转移条件并与传统的半干式消耗品相容
• 环境友好 — 环保型耗才可节省废弃物处理费用

超高的转印效率
与其他印迹技术相比较，使用 Trans-Blot® Turbo™ 系统可获得更高的灵敏度和更好的转印效率。 这一数据集显示仅使用 Trans-Blot Turbo 系统成功转印 1.25 ng 蛋白条带。 在 Criterion™ TGX™ 4–20% 凝胶上分离转铁蛋白的系列稀释并使用四种不同印迹技术进行转移。 所有图像均调整至相同设置。

在 3、7 或 10 分钟内转移
使用 Trans-Blot® Turbo™ 转印包进行快速转印
3 分钟方案 — 在短短 3 分钟内完成 Mini-PROTEAN® TGX™ 凝胶的超快速转印（蛋白分子量范围：5–150 kD）
7 分钟方案 — 在 7 分钟内高效转印 4块个小型或 2 块中型凝胶（蛋白质分子量范围：5–150 kD）
10 分钟方案 — 在 10 分钟内高效转印 4块小型或 2 块中型凝胶（蛋白质分子量范围：25–300+ kD）
大肠杆菌 裂解物 (6 µg) 稀释了两倍。 样本用 Mini-PROTEAN TGX 凝胶进行分离，用 Trans-Blot Turbo 系统进行转移，用 SYPRO Ruby 进行染色，然后在 VersaDoc™ MP 4000 系统上进行成像。 通道 1 中的标准品是 Precision Plus Protein™ 未染标准品，顶部带为 250 kD MW。

更好的转印不同类型的蛋白
2x 蛋白质转移对使用 Trans-Blot® Turbo™ 和 iBlot 系统转印的等量 2-D 凝胶进行的定量表明使用 Trans-Blot Turbo 系统转印和检测到的蛋白数增加了一倍。 在 Bio-Rad 的 ReadyStrip™ 11 cm pH 5–8 的 IPG 胶条上聚焦大鼠肝提取物，并将其分离到 AnykD™ Criterion™ TGX™ 凝胶上。 使用 Trans-Blot Turbo 和 iBlot 系统转移复制凝胶，这两种系统都使用制造商 ’ *的 7 分钟实验方案。 随后用 SYPRO Ruby 对硝化纤维素膜进行染色，并在 VersaDoc™ MP 4000 系统上进行成像。

	Trans-Blot® Turbo™ Transfer Starter System 1704155  Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit		Trans-Blot® Turbo™ Transfer System 1704150 Blotting instrument, includes base, 2 cassettes to hold 1-2 midi or up to 4 mini blotting sandwiches, blot roller
	Trans-Blot® Turbo™ Mini PVDF Transfer Packs 1704156  Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system		Trans-Blot® Turbo™ RTA Mini PVDF Transfer Kit, for 40 blots 1704272  Ready-to-assemble transfer kit includes 40 mini-sized PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks
	Trans-Blot® Turbo™ Midi PVDF Transfer Packs 1704157  Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system		Trans-Blot® Turbo™ RTA Midi PVDF Transfer Kit, for 40 blots 1704273  Ready-to-assemble transfer kit includes 40 midi-sized PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks
	Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs 1704158  Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system		Trans-Blot® Turbo™ RTA Mini LF PVDF Transfer Kit, for 40 blots 1704274  Ready-to-assemble transfer kit includes 40 mini-sized LF PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks
	Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs 1704159  Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system		Trans-Blot® Turbo™ RTA Midi LF PVDF Transfer Kit, for 40 blots 1704275  Ready-to-assemble transfer kit includes 40 midi-sized LF PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks
	Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit, for 40 blots 1704270  Ready-to-assemble transfer kit includes 40 mini-sized nitrocellulose membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks		Trans-Blot® Turbo™ Cassette 1704151  Pkg of 1, cassette, to hold 1 midi or up to 2 mini blotting sandwiches, for use with Trans-Blot Turbo transfer system
	Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit, for 40 blots 1704271  Ready-to-assemble transfer kit includes 40 midi-sized nitrocellulose membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks		Trans-Blot® Turbo™ Base 1704152  Blotting instrument base, does not include any cassettes

耗材试剂套装：

Use the Trans-Blot Turbo RTA transfer kit for fast, efficient protein transfer with the Trans-Blot Turbo rapid western blotting transfer system.

• Economical transfer option for high-throughput labs
• Optimized buffer, membrane, and filter paper combination
• PVDF membrane reduces nonspecific binding
• For 40 mini-gel transfers; requires assembly

Related Products

View our wide selection of transfer membrane sandwiches for the Trans-Blot Turbo system:

• Preassembled transfer packs for 10 transfers
• Ready-to-assemble kits for 40 transfers
• PVDF, low fluorescence (LF) PVDF, or nitrocellulose membranes

TGX凝胶订购信息：

	Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4569033 Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells		4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561093 Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
	4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561083 Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells		4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl 4561096 Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
	4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl 4561086 Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells		10% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561033 Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
	7.5% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561023 Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells		12% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561043 Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
	10% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl 4561036 Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells		12% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl 4561046 Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells